By R. Durbin (Eds.)

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1967). , 1965) and rhizobitoxine ( O w e n s and W r i g h t , 1965b). M e d i a for the production of other t o x i n s , for e x a m p l e , h e l m i n t h o s p o r o s i d e (Steiner and Strobel, 1971), P C - t o x i n (Pringle and Scheffer, 1963), H C - t o x i n (Scheffer and U l l s t r u p , 1965), and for toxins p r o duced by Corynebacterium s p p . , 1973) contained yeast extract as a source of g r o w t h factors. F. 1. Environmental Factors Temperature T o x i n production usually o c c u r s o v e r a relatively n a r r o w range of t e m p e r a t u r e s , and fermentation t e m p e r a t u r e s of about 2 1 ° - 2 8 ° C are c o m m o n l y used.

W h e n a m i x t u r e c o n t a i n i n g the material to b e purified is applied to a c o l u m n p a c k e d with the p o l y m e r - b o u n d c o m p o u n d , the material will be b o u n d to the p o l y m e r and thus will be r e m o v e d selectively from the m i x t u r e . T h e material can then be displaced from the p o l y m e r by elution of the c o l u m n with a solution c o n t a i n i n g a high c o n c e n t r a t i o n of a c o m p o u n d that h a s affinity for the b o u n d c o m p o u n d .

1952). T h e relatively n e w t e c h n i q u e , affinity c h r o m a t o g r a p h y , used for purification of proteins and nucleic a c i d s , has been applied to toxin isolation. A c o m p o u n d that has a high affinity for a specific m a c r o m o l e c u l e ( e . g . , the substrate of an e n z y m e ) is covalently attached to an insoluble p o l y m e r such as cellulose or a d e x t r a n . W h e n a m i x t u r e c o n t a i n i n g the material to b e purified is applied to a c o l u m n p a c k e d with the p o l y m e r - b o u n d c o m p o u n d , the material will be b o u n d to the p o l y m e r and thus will be r e m o v e d selectively from the m i x t u r e .

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