By Zhou Songyang (auth.), Zhou Songyang (eds.)

New and quick advances in know-how have outfitted us with a number of instruments and systems to invite basic questions of telomere legislation and feature allowed investigators to hold out experiments utilizing different version platforms. for instance, proteomic, genomic, and molecular ways have afforded us remarkable perception into the advanced protein interplay networks at paintings at the telomere chromatin and the targeted information about telomere dynamics in line with rigidity or stimuli. Telomeres and Telomerase: tools and Protocols, moment Edition builds upon the telomerase assays featured within the well known first version to surround many various assays that permit investigators to question the functionality of telomere proteins and the responses of the telomere DNA, together with special examinations of biochemical, molecular, and proteomic techniques. Written within the hugely winning Methods in Molecular Biology™ sequence layout, chapters comprise introductions to their respective themes, lists of the required fabrics and reagents, step by step, conveniently reproducible laboratory protocols, and professional tips about troubleshooting and heading off identified pitfalls.

Authoritative and sensible, Telomeres and Telomerase: equipment and Protocols, moment Edition serves as an incredible, up to date advisor for investigators extra pursing this very important box of study.

Show description

Read Online or Download Telomeres and Telomerase: Methods and Protocols PDF

Best nonfiction_3 books

The Orange Fairy Book

Andrew Lang's Fairy Books are a sequence of twenty-five collections of actual and fictional tales for kids, released among 1889 and 1913. the easiest recognized books of the sequence are the twelve collections of fairy stories, often called Andrew Lang's "Coloured" Fairy Books or Andrew Lang's Fairy Books of many colours.

The Bukidnon of Mindanao (Fieldiana, Anthropology, v.46)

Quantity: Fieldiana, Anthropology, v. forty six writer: [Chicago] Chicago common background Museum Press booklet date: 1956 matters: Bukidnon (Philippine humans) Notes: this is often an OCR reprint. there's quite a few typos or lacking textual content. There are not any illustrations or indexes. in case you purchase the final Books version of this e-book you get unfastened trial entry to Million-Books.

Alkali Activated Cenents & Concrete

The 1st English-language publication which experiences and summarizes around the globe study advances in alkali-activated cements and urban. crucial subject matters contain: uncooked fabrics and their homes for the construction of the 2 new kinds of binder the hydration and microstructure improvement of alkali-activated slag cements the mechanical houses and sturdiness of alkali-activated slag cement and urban different a variety of cementing structures and their functions similar criteria and requisites.

Additional resources for Telomeres and Telomerase: Methods and Protocols

Sample text

Recover the cell suspension in PBS. 2 mL above the cell pellet. Resuspend the cell pellet in the remaining supernatant. Hypotonic shock: Slowly add (drop-wise) the pre-warmed hypotonic solution to the resuspended cells while gently vortexing. Incubate at 37°C for 30–40 min (see Note 11). Prefixation: Add a few drops of freshly prepared fixative ethanol/acetic acid (3/1, V/V) and invert to mix. Spin the cells down at slow speed (200 rcf, 5 min). Remove most of the supernatant and resuspend the cell pellet in the remaining liquid.

While both the G and C probes can be used, the G probe generally yields better and stronger signals. 5–1 × 106 cpm/mL. The membrane is first washed in low-stringency buffer once at room temperature and once at 37°C, and then in highstringency buffer at least twice at room temperature. The stringency of the wash may be further raised by increasing the number of washes, or the temperature for high-stringency wash (to 37°C or 50°C if needed). The membrane should be checked with a Geiger counter periodically.

Wash three times 5 min in PBS 1×. 5 mg/mL in PBS) for 15 min. Quick wash in PBS 1×. Expose slides to a 306 nm UV source for 30 min. Typically, the slides are placed on a heating plate (55°C) covered with excess PBS and a coverslip (to avoid desiccation), and placed under a flipped-over UV box. The slides should be 1 cm away from the UV source. After UV exposure, immerse slides in PBS 1× to help the coverslip to come off and the slides to cool down. After draining all the PBS, treat slides with 3 U/mL of ExoIII for 5 min at room temperature (use at least 40 mL/slide and put a coverslip on to ensure even distribution) (see Note 17).

Download PDF sample

Rated 4.37 of 5 – based on 19 votes