By J.A. Callow (Eds.)
Major developments were made in pathogen detection applied sciences over the last decade. Indexing of crops and plant elements for the presence of particular pathogens has been optimum in a few circumstances for averting and/or controlling ailment. the recent applied sciences for detecting low degrees of pathogens increases the price of indexing as a device for plant ailment regulate. offering an outline of the prestige of detection expertise, this quantity is directed not just to scientists and scholars attracted to detection expertise, but additionally to these drawn to formulating and enforcing affliction regulate and quarantine laws. This ebook offers a conceptual framework which offers the present clinical literature, state of the art checks, and speculations on destiny advancements and specifications of pathogen indexing tools. Chapters disguise different pathogen teams, assessment present practices in parts the place detection expertise has turn into vital, and supply views on how indexing applied sciences should be utilized, how good it has labored, and which difficulties stay. Statistical remedy of detection limits, sampling concepts, probability evaluation, expense, standardization, and qc also are lined.
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Extra info for Pathogen Indexing Technologies
W. Pemberton common diseases these schemes are designed to combat, examination under the electron microscope or in a routine ELISA usually will be adequate. However, in the future it is anticipated that PCR will play an increasingly important role here. Smooth and rapid throughput of samples at minimum cost is essential and because the ELISA can be largely automated, it is particularly suitable for this application (Torrance and Jones, 1981). 2. Indexing for Nuclear Stock Production The concept of indexed nuclear stock as starting material for healthy stock schemes developed in the late 1940s when clones of virus-tested raspberries were introduced into the Scottish raspberry certification scheme (Harris and Cadman, 1949; Ebbels, 1979).
Pemberfon suited for use in routine testing for plant viruses. Plant Pathology 30, 1-24. , Kersters, K. and Swings, J. (1995). Reclassification of Xanthomonas. International Journal of Systematic Bacteriology 45,472-489. Vivian, A. (1992). Identification of plant pathogenic bacteria using nucleic acid technology. In 'Techniques for the Rapid Detection of Plant Pathogens'. M. , eds) pp 145-161. Blackwell Scientific Publications, Oxford. , Fisher, G. (1986). Studies on the relationship of contamination of seed potato tubers with Erwinia spp.
The role of international culture collections as sources of well-documented reference strains and for deposition of new and noteworthy strains is of vital importance. , Delhotal, P. and Fayet, J. (1990). An expert system for diagnosis of post-harvest potato diseases. EPPO Bulletin 20, 341-347. M. and Cambra, M. (1994). Comparison of immunological methods for detection of Erwiniu curotouoru subsp. utrosepticu using monoclonal and polyclonal antibodies. , Rupolph, K. , eds). pp 423. INRA Editions, Versailles.